Automated On-line Sampling System

In the processes of cell cultivation and microbial fermentation, control of critical process parameters plays a vital role in ensuring successful experiment or production. Unlike conventional cultivation equipment that usually can only measure and control several common process parameters such as pH, DO, temperature, speed, etc., Jing Liang JL-YGZ600 automated on-line sampling system is capable of real-time controlling metabolites and calculating nutrient consumption in cell cultivation and microbial fermentation process, allowing user to precisely get information about the status of cell culture while achieving traceability.

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System Components

The automated on-line sampling system is mainly composed of three parts:
Automatic on-line sampling
Automatic biochemical analysis
Intelligent feedback control of fed-batch

Automated On-line Sampling System
Analysis Parameters of Sampling
Item Range (not prediluted) Range (prediluted)
Glucose 0.05~6g/L 0.05~60g/L
Lactic acid 0.05~5g/L 0.05~50g/L
Glutamate 15~1460mg/L 15~14600mg/L
Glutamine 15~1460mg/L 15~14600mg/L
Glycerin 0.05~2g/L 0.05~20g/L
Methanol 0.02~0.5g/L 0.01~10g/L
Ethanol (alcohol content) 0.02~1g/L 0.04~20g/L
Lysine 0.0~1.5g/L 0.0~15g/L
Xylose 0.05~2g/L 0.05~20g/L
Galactose 0.1~2g/L 0.1~20g/L
pH 6.0~8
Ammonium ion 0.01~0.6g/L 0.01~6g/L
Sodium ion 20.0~200mmol/L
Potassium ion 0.5~15mmol/L
Calcium ion 0.1~5mmol/L
Cases
    1. Automated On-line Sampling System
    2. Fermentation Process Research and Development Project of Zhejiang SDSW Research Institute
      • Project: Vitamin B2
      • Reaction: 96 hours
      • Item: glucose, lactic acid
      • Testing time: 10min
      • Object: 5L bioreactor
      • Result: workers’ day and night shifts are cancelled during the cell culture process, feeding accuracy and product expression rate are improved by high-frequency sampling testing and feedback controlling of fed-batch.
    1. Automated On-line Sampling System
    2. Cervical Cancer Vaccine Project of Jiangsu RKSW Pharmaceutical Technology Co., Ltd.
      • Project: vaccine for cervical cancer
      • Reaction: 80 hours
      • Item: glycerin
      • Testing time: 20min
      • Object: three 30L bioreactors
      • Result: workers’ day and night shifts are cancelled during the cell culture process, feeding accuracy and product expression rate are improved by high-frequency sampling testing and feedback controlling of fed-batch.
    1. Automated On-line Sampling System
    2. YGF300 biochemistry analyzer
      Performance Parameters:
      • Sampling frequency and sampling rate can be set according to actual needs
      • Fully automatic aseptic operation in sampling process
      • Sampling rate: 2-10 ml/min
      • Sampling interval: Min. 5min
      • Sample volume: 0.5-5 ml